rabbit anti human idh2  (Cell Signaling Technology Inc)

Journal: Frontiers in Physiology

Article Title: Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals

doi: 10.3389/fphys.2021.773995

Figure Lengend Snippet: Alterations in select mitochondrial enzymes with CON vs. VAR training. Data presented in arbitrary units (ADUs) include isocitrate dehydrogenase 2 (IDH2) protein levels from the sarcoplasmic protein pool (panel A ), carnitine palmitoyltransferase 1 (CPT1) protein levels from the sarcoplasmic protein pool (panel B ), and very long-chain specific acyl-CoA dehydrogenase (VLCAD) protein levels from the sarcoplasmic protein pool (panel C ). Data are presented as means with standard deviation bars. Panel (D) contains representative Western blot images for each target. Note that two of Ponceau images in panel (D) (for IDH2 and VLCAD) are the same given that the same subject was used for the representative Western blot images of these targets.

Article Snippet: Membranes were then incubated overnight at 4°C with the following antibody cocktails (each at a 1:1,000 dilution) in TBST with 5% bovine serum albumin (BSA): (i) goat anti-human creatine kinase (CKM; Abcam; Cambridge, MA, United States; cat# ab174672), (ii) rabbit anti-human L-type amino acid transporter (LAT1, Cell Signaling Technology; Danvers, MA, United States; cat# 5347), (iii) rabbit anti-human glucose transporter 4 (GLUT4; Cell Signaling Technology, cat# 2213), (iv) rabbit anti-human lactate dehydrogenase (LDHA; Cell Signaling Technology, cat# 2012), (v) rabbit anti-human phosphofructokinase (PFKM; Abcam, cat#: ab154804), (vi) rabbit anti-human total oxidative phosphorylation (OXPHOS) cocktail (Abcam, cat# ab110411), (vii) rabbit anti-human complex IV (Cell Signaling Technology, cat# 4850), (viii) rabbit anti-human isocitrate dehydrogenase 2 (IDH2; Cell Signaling Technology, cat# 56439), (ix) rabbit anti-human acyl-CoA dehydrogenase very long chain (VLCAD; Abcam, cat# ab188872), (x) rabbit anti-human carnitine palmitoyltransferase I (CPTI; Abcam, cat# ab134135), and (xi) rabbit anti-human hexokinase II (HK2; Cell Signaling Technology, cat# 2867).

Techniques: Standard Deviation, Western Blot

Journal: Molecular Systems Biology

Article Title: STAT 3 ‐dependent analysis reveals PDK 4 as independent predictor of recurrence in prostate cancer

doi: 10.15252/msb.20199247

Figure Lengend Snippet: SDHB protein expression levels in a TMA ( n = 83) detected by immunohistochemistry (IHC). Box‐plot shows median, 1 st and 3 rd quartiles, and whiskers extend to ± 1.5 interquartile range. Jitter represents single values in groups. Kruskal–Wallis test and Dunn's all‐pairs test were applied. Q, adj. P ‐value; n.s., not significant. TMA, tissue microarray. Representative IHC staining of SDHB in normal prostate glands and Gleason grade (GL) 3–5 PCa glands. Scale bar = 100 μm. IDH2 protein expression levels in a TMA ( n = 83) detected by IHC. Box‐plot shows median, 1 st and 3 rd quartiles, and whiskers extend to ± 1.5 interquartile range. Jitter represents single values in groups. Kruskal–Wallis test and Dunn's all‐pairs test were applied. Q, adj. P ‐value; n.s., not significant. Representative IHC staining of IDH2 in normal prostate glands and GL 3–5 PCa glands. Scale bar = 100 μm. Nuclear (N) and cytoplasmic (C) STAT3 protein expression levels in a TMA ( n = 83) detected by IHC. Box‐plot shows median, 1 st and 3 rd quartiles, and whiskers extend to ± 1.5 interquartile range. Jitter represents single values in groups. Kruskal–Wallis test and Dunn's all‐pairs test were applied. Q = adj. P ‐value; n.s., not significant. Source data are available online for this figure.

Article Snippet: Rabbit Anti‐Human IDH2, polyclonal, 1:100 dilution IHC , Proteintech Group , Cat# 15932‐1‐AP, RRID:AB_2264612.

Techniques: Expressing, Immunohistochemistry, Microarray

Journal: Molecular Systems Biology

Article Title: STAT 3 ‐dependent analysis reveals PDK 4 as independent predictor of recurrence in prostate cancer

doi: 10.15252/msb.20199247

Figure Lengend Snippet: A–F Time to BCR in months for STAT3 (A), HIF‐1α (B), c‐MYC (C), CNOT1 (D), IDH2 (E), and SDHB (F) in the MSKCC PCa GSE21032 data set. Groups were generated by a median split. P ‐values were estimated by log‐rank test and adjusted with Benjamini–Hochberg method. + = censored.

Article Snippet: Rabbit Anti‐Human IDH2, polyclonal, 1:100 dilution IHC , Proteintech Group , Cat# 15932‐1‐AP, RRID:AB_2264612.

Techniques: Generated

Journal: Molecular Systems Biology

Article Title: STAT 3 ‐dependent analysis reveals PDK 4 as independent predictor of recurrence in prostate cancer

doi: 10.15252/msb.20199247

Figure Lengend Snippet: STAT3‐ and PDK4 ‐stratified subgroups were generated by median splits in MSKCC PCa GSE21032 data set. Pearson correlation between STAT3 and PDK4 is shown. Kaplan–Meier plot shows stratified subgroups. P ‐values were estimated by log‐rank test and adjusted with Benjamini–Hochberg method. Hi, high; lo, low. Western blot of STAT3, PDK4, and β‐TUBULIN proteins in 22Rv1 cells with or without knockdown of STAT3. Ctrl, scrambled control; shSTAT3, short hairpin knockdown of STAT3 . ChIP assay from IL‐6‐stimulated or non‐stimulated 22Rv1 cells with or without knockdown of STAT3 was immunoprecipitated with a STAT3‐specific antibody (blue shades) and IgG antibody as a negative control (orange shades) followed by qPCR with a promoter‐specific primer pair for the PDK4 gene. Bars represent mean ± SD from two technical replicates. Precipitated DNA is presented as % of input. One representative experiment is shown. Result of qPCR using primer pair 2 is shown. Ctrl = scrambled control; shSTAT3 = short hairpin knockdown of STAT3; +IL‐6 = IL‐6‐stimulated. Correlation of STAT3, c‐MYC , and HIF‐1α with PDK1‐4 , PDC genes ( PDHA1, PDHB, PDHX, DLAT, DLD ) and TCA/OXPHOS genes (CS, IDH2, IDH3A, SDHB, SDHC, ATP5A1, NDUFS1) in MSKCC PCa (GSE21032). Dot colors represent Pearson correlation (1 = red; −1 = blue); dot sizes represent adj. P ‐values ≤ 0.05. Only significant correlations are shown. P ‐values were adjusted with Benjamini–Hochberg method. Source data are available online for this figure.

Article Snippet: Rabbit Anti‐Human IDH2, polyclonal, 1:100 dilution IHC , Proteintech Group , Cat# 15932‐1‐AP, RRID:AB_2264612.

Techniques: Generated, Western Blot, Knockdown, Control, Immunoprecipitation, Negative Control

Journal: Molecular Systems Biology

Article Title: STAT 3 ‐dependent analysis reveals PDK 4 as independent predictor of recurrence in prostate cancer

doi: 10.15252/msb.20199247

Figure Lengend Snippet:

Article Snippet: Rabbit Anti‐Human IDH2, polyclonal, 1:100 dilution IHC , Proteintech Group , Cat# 15932‐1‐AP, RRID:AB_2264612.

Techniques: Recombinant, Sequencing, Software, Bicinchoninic Acid Protein Assay, Mass Spectrometry

Journal: Molecular Systems Biology

Article Title: STAT 3 ‐dependent analysis reveals PDK 4 as independent predictor of recurrence in prostate cancer

doi: 10.15252/msb.20199247

Figure Lengend Snippet:

Article Snippet: Rabbit Anti‐Human IDH2, polyclonal, 1:100 dilution IHC , Proteintech Group , Cat# 15932‐1‐AP, RRID:AB_2264612.

Techniques: Incubation